Leucine Dehydrogenase (LeuDH)
Tuairisgeul goirid:
Mu Leucine dehydrogenase
Tha 1 seòrsa de thoradh enzyme LeuDH (Àireamh mar ES-LeuDH-101) air a leasachadh le SyncoZymes.Faodaidh LeuDH ammoniation lughdachail de dh’ aigéad 4-methylbutanoic (salann) a ghlacadh gu L-leucine no oxidation leucine gu searbhag 4-methylbutanoic.Faodaidh ES-LeuDH cuideachd amination lughdachail de α-keto acids a bhrosnachadh le structar coltach ris gus derivatives aminoideach co-fhreagarrach ullachadh.Ann am pròiseas catalysis, tha feum air NADH mar neach-còmhdhail hydrogen.
Seòrsa ath-bhualadh leucine dehydrogenasecatalytic
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Post-d:lchen@syncozymes.com
| Einnseanan | Còd Bathar | Sònrachadh |
| Pùdar Enzyme | ES-LeuDH-101 | seata de 1 Monoamine Oxidase, 50 mg gach 1 rud * 50mg / item, no meud eile |
Faodar an siostam freagairt a leanas a chleachdadh airson iomradh:
1-10 mg / ml: fo-strat
10 mg/ml: enzyme
10% (v/v): Cosolvent organach (1)
100 mM: bufair fosfáit (pH7.0)
0.2 mg/ml: NAD+
100 mM: cruth ammonium
10 mg / ml: cruth dehydrogenase (FDH)
Thoir a-steach na h-ath-bheachdan aig crathadh teòthachd seasmhach (mar eisimpleir, 30oC, 150rpm) a-steach airson 24 ~ 48 uairean.Centrifuge gach measgachadh gus pròtain a dhùsgadh, agus faodar an supernatant a chleachdadh airson mion-sgrùdadh TLC, HPLC no GC.
Nota (1): Tha fuasgladh organach (leithid DMSO, methanol, acetonitrile, IPA, msaa) roghainneil, air a chleachdadh airson fo-stratan do-sholabailte.
